plasmid pcdna3 flag rheb n153t (Addgene inc)
Structured Review
Plasmid Pcdna3 Flag Rheb N153t, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+flag+rheb+n153t/pcDNA3-FLAG-Rheb-N153T+(Plasmid+%2319997)/pmc09648415-88-20-22
Average 91 stars, based on 14 article reviews
Images
Related Articles
Transfection:Article Title: VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle Article Snippet: The cells were serum starved (no serum in the growth media) in Hanks’ Balanced Salt Solution (HBSS; Invitrogen, 14025076) for 1 h then restimulated with the DMEM-FBS full medium containing 10% FBS. .. For transfection, U2OS cells were cultured on coverslips and transfected with 1 μg each of Article Title: Mitochondria are required for pro‐ageing features of the senescent phenotype Article Snippet: .. For the overexpression of activated Rheb and PGC‐1β, MEFs were transfected with an empty vector and Cell Culture:Article Title: VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle Article Snippet: The cells were serum starved (no serum in the growth media) in Hanks’ Balanced Salt Solution (HBSS; Invitrogen, 14025076) for 1 h then restimulated with the DMEM-FBS full medium containing 10% FBS. .. For transfection, U2OS cells were cultured on coverslips and transfected with 1 μg each of Plasmid Preparation:Article Title: VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle Article Snippet: The cells were serum starved (no serum in the growth media) in Hanks’ Balanced Salt Solution (HBSS; Invitrogen, 14025076) for 1 h then restimulated with the DMEM-FBS full medium containing 10% FBS. .. For transfection, U2OS cells were cultured on coverslips and transfected with 1 μg each of Article Title: Regulation of mTORC1 by lysosomal calcium and calmodulin Article Snippet: Human GAPDH VIC (Hs02758991_g1) was used as an endogenous control. .. Myc-mTOR (Addgene plasmid # 1861), pRK5-HA GST RagA 66L (Addgene plasmid # 19300), pRK5-HA GST RagC 75L (Addgene plasmid # 19305) and HA GST PreScission p70 S6K1 (Addgene plasmid # 15511) were gifts from David Sabatini. Control:Article Title: VCP maintains lysosomal homeostasis and TFEB activity in differentiated skeletal muscle Article Snippet: The cells were serum starved (no serum in the growth media) in Hanks’ Balanced Salt Solution (HBSS; Invitrogen, 14025076) for 1 h then restimulated with the DMEM-FBS full medium containing 10% FBS. .. For transfection, U2OS cells were cultured on coverslips and transfected with 1 μg each of Stable Transfection:Article Title: Acid suspends the circadian clock in hypoxia through inhibition of mTOR Article Snippet: .. U2OS Arntl ::dLUC lines stably expressing constitutively active RHEB ( Urano et al., 2005 ) where similarly created by seeding 200,000 cells in a 6-well dish and the following day transfecting with 0.5 ng of Expressing:Article Title: Acid suspends the circadian clock in hypoxia through inhibition of mTOR Article Snippet: .. U2OS Arntl ::dLUC lines stably expressing constitutively active RHEB ( Urano et al., 2005 ) where similarly created by seeding 200,000 cells in a 6-well dish and the following day transfecting with 0.5 ng of Sequencing:Article Title: Acid suspends the circadian clock in hypoxia through inhibition of mTOR Article Snippet: .. U2OS Arntl ::dLUC lines stably expressing constitutively active RHEB ( Urano et al., 2005 ) where similarly created by seeding 200,000 cells in a 6-well dish and the following day transfecting with 0.5 ng of Over Expression:Article Title: Mitochondria are required for pro‐ageing features of the senescent phenotype Article Snippet: .. For the overexpression of activated Rheb and PGC‐1β, MEFs were transfected with an empty vector and Pyrolysis Gas Chromatography:Article Title: Mitochondria are required for pro‐ageing features of the senescent phenotype Article Snippet: .. For the overexpression of activated Rheb and PGC‐1β, MEFs were transfected with an empty vector and |
